Proteomics Instrumentation

Thermo mass spectrometer

Thermo Orbitrap Ascend and Orbitrap Fusion Tribrid mass spectrometers with quadrupole, linear ion trap and orbitrap detectors with CID and HCD fragmentation for use with LC-MS, LC-MS/MS, and intact protein analysis. The Orbitrap Ascend has ETD and PTCR fragmentation and High Mass Range to m/z 16,000. Vanquish Neo for micro flow to nano flow and Ultimate 3000 RSLCnano UPLC for nanoflow ultra high pressure liquid chromatography. Evosep One high throughput chromatography system.

Proteomics Software:

Proteome Discoverer 2.5 and 3.1 database search algorithmsfor protein identification, quantitation, and modification database search algorithms; Spectronaut for DIA identification and quantitation, Scaffold 5 Q+ and PTM data visualization and validation software, and Byonic for protein modification analysis.


Protein and peptide MW determination. MS/MS and MSn of peptides with CID/HCD/ETD/PTCR. Protein identification from gel bands, gel sections, and complex mixtures. Protein quantitation from spectral counting, stable isotope labeling, and intensity based quantitation, or data independent acquisition. PTM analysis and characterization. Native protein mass spectrometry with high mass range and charge state reduction using PTCR. High throughput proteomics for large scale applications with Evosep One.