Confocal and Super Resolution Microscopes

The Zeiss LSM 710/Elyra S.1 has a single inverted microscope stand that functions as both a laser scanning confocal and a structured illumination super resolution microscope.

The shared Zeiss Axiovert microscope stand includes:

• Objectives:
  • EC Plan-Neofluor 10X; NA = 0.30, WD = 5.2mm
  • Plan-Apochromat 20X; NA = 0.80, WD = 0.55mm
  • Plan-Apo 40X (oil); NA = 1.4, WD = 0.13 mm
  • Plan-Apo 63X (oil); NA = 1.4, WD = 0.19 mm
  • Water 63X (oil); NA = 1.2, WD = 0.28 mm
• Incubation: The microscope has incubation controls for both temperature and CO₂ for live cell imaging.

The Zeiss LSM 710 confocal microscope can be used for fluorescence imaging of a variety of biological and material samples. The instrument provides high resolution images using a variable pinhole for optical 'Z' sectioning and laser scanning. The system is equipped with the following excitation wavelengths: 405nm, 458nm, 488nm, 514nm, 561nm and 633nm. The filterless scanhead allows for tunable emission detection of a wide array of fluorescent molecules.

Advanced imaging techniques, including spectral imaging, FRET and FRAP, are available on this instrument.

The Zeiss Elyra S.1 structured illumination super resolution system achieves a lateral resolution of ~100nm and an axial resolution of ~200nm through the use of SR-SIM. This system has the ability to penetrate up to 20um into the tissue and can acquire Z-stack, multi-channel SR-SIM data with up to 4 channels. The system is equipped with 4 excitation laser wavelengths for standard fluorophores (405nm, 488nm, 561nm and 633nm) and comes with emission filters for DAPI, GFP, RFP and Alexa 633.

Location: This microscope is located in MBB 1.426B.

Instrument Access: Assisted use and training for individual use are available. To schedule assisted use or training, please email Paul Oliphint.

Fees: trained users: $53/hour; assisted use: $123/hour

The Nikon A1R confocal microscope provides high-resolution fluorescence imaging using a variable pinhole for optical Z sectioning and either galvano or high-speed resonant scanning (30-420 fps). The system is equipped with the following excitation wavelengths: 405, 445, 488, 514, 561, and 640 nm, as well as filter sets for DAPI, CFP, GFP, YFP, TRITC, and Cy5. It also has ultra-sensitive GaAsP and high sensitivity PMT detectors and Perfect Focus control, which maintains the focal plane over long imaging experiments.

Objectives:

• Plan Apo λ 10x; NA - 0.45; WD - 4 mm
• Plan Apo λ 20x; NA - 0.75; WD - 1 mm
• Plan Fluor DIC 40x (Oil); NA - 1.3; WD - 0.24 mm
• Plan Apo λ 60x (Oil); NA - 1.4; WD - 0.14 mm

Incubation: An incubation stage for temperature and CO₂ control for long-term live cell imaging experiments is available.

Location: This microscope is located in HDB 3.222KB.

Instrument Access: Assisted use and training for individual use are available. To schedule assisted use or training, please email Anna Webb.

Fees: trained users: $53/hour; assisted use: $123/hour

The Nikon W1 spinning disk confocal microscope uses a Yokogawa W1 scanning head with multiple pinholes for ultrafast confocal imaging. The W1 has an extra wide field of view, making it ideal for imaging large specimens. It has the following excitation lasers: 405, 488, 561, and 640 nm, and filters for DAPI, GFP, Texas Red, and Cy5. It is equipped with dual monochromatic Andor EMCCD cameras for simultaneous two-color imaging. It also has Perfect Focus control and DIC optics.

Objectives:

• Plan Fluor DLL Ph1 10x; NA - 0.3; WD - 16.0 mm
• Super Plan Fluor Ph1 ELWD 20x; NA - 0.45; WD - 6.9-8.2 mm
• Plan Apochromat λ 20x; NA - 0.75; WD - 1.0 mm
• Super Plan Fluor ELWD 40x; NA - 0.6; WD - 2.8-3.6 mm
• Plan Apochromat VC 60x (Water); NA - 1.20; WD - 0.28-0.31 mm
• Plan Apochromat λ 100x (Oil); NA - 1.45; WD - 0.13 mm

Incubation: An incubation stage for temperature and CO₂ control for long-term live cell imaging experiments is available.

Location: This microscope is located in MBB 1.426T.

Instrument Access: Assisted use and training for individual use are available. To schedule assisted use or training, please email Paul Oliphint.

Fees: trained users: $53/hour; assisted use: $123/hour

The TIRF/N-STORM System has a single inverted microscope stand that functions as both a TIRF microscope and super resolution N-STORM system.

The shared Nikon Eclipse Ti-2 microscope stand includes:

• Objectives:
  • Plan Apochromat λ 10x; NA - 0.45; WD - 4.0 mm
  • Plan Apochromat λ 20x; NA - 0.75; WD - 1.0 mm
  • Plan Fluor 40x (Oil); NA - 1.3; WD - 0.20 mm
  • Plan Apochromat λ 60x (Oil); NA - 1.4; WD - 0.13 mm
  • High Power TIRF Auto Correction Collar 100x (Oil); NA - 1.49; WD - 0.12 mm
• Incubation: The microscope has incubation controls for both temperature and CO₂ for live cell imaging.
• Focus control: The microscope is equipped with Perfect Focus to automatically correct focus drift.

The Total Internal Reflectance Fluorescence (TIRF) microscope provides high signal-to-noise fluorescence imaging of a thin plane at the cell-coverslip interface. This microscope is equipped with filters for DAPI, GFP, Texas Red, and Cy5. It has two monochromatic EMCCD cameras that can be used for single color imaging or simultaneous two color imaging. It is equipped with the following laser excitation wavelengths: 405, 488, 561, and 640 nm.

The N-STORM (Stochastic Optical Reconstruction Microscopy) system uses a super resolution technique that images individual, blinking fluorophores over time and pinpoints the precise location of each fluorophore to reconstruct the image. It can achieve a lateral resolution limit of ~20 nm and axial resolution of ~50 nm. Multicolor imaging and 3D localization are possible with this microscope. This microscope is equipped with filters for DAPI, GFP, Texas Red, and Cy5. It uses a Hamamatsu sCMOS camera for high sensitivity detection of fluorophores. It is also equipped with the following laser excitation wavelengths: 405, 488, 561, and 640 nm.

Location: This microscope is located in MBB 1.426T.

Instrument Access: Assisted use and training for individual use are available. To schedule assisted use or training, please email Anna Webb.

Fees: trained users: $53/hour; assisted use: $123/hour